Tribulus (Tribulus terrestris L.) is an herbal plant, also known as caltrop and puncture vine. It’s been used in traditional medicine for a variety of potential health benefits such as pain, inflammation, heart-related problems, and high blood pressure. It’s also promoted for general well-being, vitality, and mood. Today it’s commonly found as an ingredient in dietary supplements marketed to boost testosterone and enhance sexual (libido) and athletic performance.

No strong evidence supports the marketed claims that Tribulus can enhance sexual and athletic performance. Its safety as a dietary supplement ingredient is not yet fully understood.

The dietary supplement ingredient Tribulus is prepared from the leaves, root, and fruit of the Tribulus terrestris L. plant, which contains numerous chemical compounds, including steroidal saponins (plant steroids). The plant itself is known to be toxic to rats and sheep after ingesting large amounts. Effects include damage to the heart, liver, and kidneys.

Is Tribulus safe for humans?

Research evaluating the safety of Tribulus in humans is limited. More research is needed to determine the safety of Tribulus as a dietary supplement ingredient, especially long term and in varying doses.

  • Short-term studies (up to 3 months) have reported few adverse effects, such as stomach cramps and nausea.
  • Sleep disturbances, exhaustion, fatigue, and elevated heart rate have been reported after consuming more than 1,000 mg per day.
  • A few cases of severe liver and renal (kidney) damage have been reported after individuals consumed supplements containing Tribulus.

What are the effects of Tribulus on athletic performance?

Some small-scale studies have examined the effect of Tribulus on athletic performance in populations such as rugby players and CrossFit® athletes. Overall, these studies do not show any significant improvements in body composition (muscle or body mass), strength, or exercise performance.

What are the effects of Tribulus on sexual performance?

Some studies suggest that Tribulus might increase testosterone levels and improve erectile and sexual dysfunction. However, clinical trials involving men report mixed results when Tribulus was used to increase testosterone levels or improve erectile dysfunction. Likewise, some clinical trials suggest that Tribulus might improve sexual function for women suffering from low libido or hypoactive sexual desire disorder (HSDD). Overall, however, larger and more in-depth studies are needed to confirm any of these effects in humans.

Will Tribulus cause a positive drug test? Is it prohibited for Service Members?

Tribulus is not on the DoD Prohibited Dietary Supplement Ingredients List, and it shouldn’t cause a positive result on a routine military drug test. When considering any dietary supplement product, look for those displaying a third-party certification seal.

 

Updated 3 March 2024

References

Abudayyak, M., Jannuzzi, A. T., Özhan, G., & Alpertunga, B. (2014). Investigation on the toxic potential of Tribulus terrestris in vitro. Pharmaceutical Biology, 53(4), 469–476. doi:10.3109/13880209.2014.924019

Akhtari, E., Raisi, F., Keshavarz, M., Hosseini, H., Sohrabvand, F., Bioos, S., . . . Ghobadi, A. (2014). Tribulus terrestris for treatment of sexual dysfunction in women: Randomized double-blind placebo - controlled study. DARU Journal of Pharmaceutical Sciences, 22(1). doi:10.1186/2008-2231-22-40

Antonio, J., Uelmen, J., Rodriguez, R., & Earnest, C. (2000). The effects of Tribulus terrestris on body composition and exercise performance in resistance-trained males. International Journal of Sport Nutrition and Exercise Metabolism, 10(2), 208–215. doi:10.1123/ijsnem.10.2.208

Aoki, T., Silva, G., Reis, B., Yamada, S., Lima, S., & Postigo, S. (2016). Assessment of the effects of Tribulus terrestris on sexual function of menopausal women. Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics, 38(03), 140–146. doi:10.1055/s-0036-1571472

Borrelli, F., Colalto, C., Delfino, D. V., Iriti, M., & Izzo, A. A. (2018). Herbal dietary supplements for erectile dysfunction: A systematic review and meta-analysis. Drugs, 78(6), 643–673. doi:10.1007/s40265-018-0897-3

Campanelli, M., De Thomasis, R., & Tenaglia, R. L. (2016). Priapism caused by 'Tribulus terrestris'. International journal of impotence research, 28(1), 39–40. https://doi.org/10.1038/ijir.2015.30

de Souza, K. Z. D., Vale, F. B. C., & Geber, S. (2016). Efficacy of Tribulus terrestris for the treatment of hypoactive sexual desire disorder in postmenopausal women: A randomized, double-blinded, placebo-controlled trial. Menopause, 23(11), 1252–1256. doi:10.1097/gme.0000000000000766

Diseases, N. I. o. D. a. D. a. K. (2022). LiverTox: Clinical and Research Information on Drug-Induced Liver Injury: Tribulus. From https://www.ncbi.nlm.nih.gov/books/NBK583201/

Fernández-Lázaro, D., Fernandez-Lazaro, C. I., Seco-Calvo, J., Garrosa, E., Adams, D. P., & Mielgo-Ayuso, J. (2022). Effects of Tribulus terrestris L. on sport and health biomarkers in physically active adult males: A systematic review. International Journal of Environmental Research and Public Health, 19(15). doi:10.3390/ijerph19159533

Fernández-Lázaro, D., Mielgo-Ayuso, J., del Valle Soto, M., Adams, D. P., González-Bernal, J. J., & Seco-Calvo, J. (2021). The effects of 6 weeks of Tribulus terrestris L. supplementation on body composition, hormonal response, perceived exertion, and CrossFit® performance: A randomized, single-blind, placebo-controlled study. Nutrients, 13(11). doi:10.3390/nu13113969

Fernández-Lázaro, D., Seco-Calvo, J., Pascual-Fernández, J., Domínguez-Ortega, C., Del Valle Soto, M., & Mielgo-Ayuso, J. (2022). 6-week supplementation with Tribulus terrestris L. to trained male CrossFit® athletes on muscle, inflammation, and antioxidant biomarkers: A randomized, single-blind, placebo-controlled trial. International Journal of Environmental Research and Public Health, 19(23). doi:10.3390/ijerph192316158

GamalEl Din, S. F., Abdel Salam, M. A., Mohamed, M. S., Ahmed, A. R., Motawaa, A. T., Saadeldin, O. A., & Elnabarway, R. R. (2018). Tribulus terrestris versus placebo in the treatment of erectile dysfunction and lower urinary tract symptoms in patients with late-onset hypogonadism: A placebo-controlled study. Urologia Journal, 86(2), 74–78. doi:10.1177/0391560318802160

Kamenov, Z., Fileva, S., Kalinov, K., & Jannini, E. A. (2017). Evaluation of the efficacy and safety of Tribulus terrestris in male sexual dysfunction—A prospective, randomized, double-blind, placebo-controlled clinical trial. Maturitas, 99, 20–26. doi:10.1016/j.maturitas.2017.01.011

Leisegang, K., & Finelli, R. (2021). Alternative medicine and herbal remedies in the treatment of erectile dysfunction: A systematic review. Arab Journal of Urology, 19(3), 323–339. doi:10.1080/2090598x.2021.1926753

Ma, Y., Guo, Z., & Wang, X. (2017). Tribulus terrestris extracts alleviate muscle damage and promote anaerobic performance of trained male boxers and its mechanisms: Roles of androgen, IGF-1, and IGF binding protein-3. Journal of Sport and Health Science, 6(4), 474–481. doi:10.1016/j.jshs.2015.12.003

Martimbianco, A. L. C., Pacheco, R. L., Vilarino, F. L., de Oliveira Cruz Latorraca, C., Torloni, M. R., & Riera, R. (2020). Tribulus terrestris for female sexual dysfunction: A systematic review. Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics, 42(07), 427–435. doi:10.1055/s-0040-1712123

Neychev, V., & Mitev, V. (2016). Pro-sexual and androgen enhancing effects of Tribulus terrestris L.: Fact or fiction. Journal of Ethnopharmacology, 179, 345–355. doi:10.1016/j.jep.2015.12.055

Neychev, V. K., & Mitev, V. I. (2005). The aphrodisiac herb Tribulus terrestris does not influence the androgen production in young men. Journal of Ethnopharmacology, 101(1-3), 319–323. doi:10.1016/j.jep.2005.05.017

Qureshi, A., Naughton, D. P., & Petroczi, A. (2014). A systematic review on the herbal extract Tribulus terrestris and the roots of its putative aphrodisiac and performance enhancing effect. Journal of Dietary Supplements, 11(1), 64–79. doi:10.3109/19390211.2014.887602

Roaiah, M. F., Elkhayat, Y. I., GamalEl Din, S. F., & Abd El Salam, M. A. (2016). Prospective analysis on the effect of botanical medicine (Tribulus terrestris) on serum testosterone level and semen parameters in males with unexplained infertility. Journal of Dietary Supplements, 14(1), 25–31. doi:10.1080/19390211.2016.1188193

Rogerson, S., Riches, C. J., Jennings, C., Weatherby, R. P., Meir, R. A., & Marshall-Gradisnik, S. M. (2007). The effect of five weeks of Tribulus terrestris supplementation on muscle strength and body composition during preseason training in elite rugby league players. The Journal of Strength and Conditioning Research, 21(2). doi:10.1519/r-18395.1

Ryan, M., Lazar, I., Nadasdy, G. M., Nadasdy, T., & Satoskar, A. A. (2015). Acute kidney injury and hyperbilirubinemia in a young male after ingestion of Tribulus terrestris. Clinical Nephrology, 83 (2015)(03), 177–183. doi:10.5414/cn108324

Sanagoo, S., Sadeghzadeh Oskouei, B., Gassab Abdollahi, N., Salehi-Pourmehr, H., Hazhir, N., & Farshbaf-Khalili, A. (2019). Effect of Tribulus terrestris L. on sperm parameters in men with idiopathic infertility: A systematic review. Complementary Therapies in Medicine, 42, 95–103. doi:10.1016/j.ctim.2018.09.015

Saudan, C., Baume, N., Emery, C. A., Strahm, E., & Saugy, M. (2008). Short term impact of Tribulus terrestris intake on doping control analysis of endogenous steroids. Forensic Science International, 178(1), e7–e10. doi:10.1016/j.forsciint.2008.01.003

Sha'ari, N., Sy-Cherng Woon, L., Sidi, H., Das, S., Bousman, C. A., & Mohamed Saini, S. (2021). Beneficial effects of natural products on female sexual dysfunction: A systematic review and meta-analysis. Phytomedicine, 93. doi:10.1016/j.phymed.2021.153760

Shaheen, G., Akram, M., Jabeen, F., Ali Shah, S. M., Munir, N., Daniyal, M., . . . Khan, M. (2019). Therapeutic potential of medicinal plants for the management of urinary tract infection: A systematic review. Clinical and Experimental Pharmacology and Physiology, 46(7), 613–624. doi:10.1111/1440-1681.13092

Siddiqui, M. A., Itrat, M., Mobeen, A., & Khan, M. I. (2021). Efficacy of khār-i-khasak (Tribulus terrestris Linn.) in prehypertension: a randomized, double-blind, placebo-controlled trial. Journal of Complementary and Integrative Medicine, 18(4), 783–789. doi:10.1515/jcim-2020-0322

Talasaz, A. H., Abbasi, M. R., Abkhiz, S., & Dashti-Khavidaki, S. (2010). Tribulus terrestris-induced severe nephrotoxicity in a young healthy male. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 25(11), 3792–3793. https://doi.org/10.1093/ndt/gfq457

Talemi, M. N. P. E., Ardakani, S. M. P., & Roozbeh, B. (2021). Tribulus terrestris may decrease muscle damage markers following a high-intensity resistance exercise: A pilot study. International Journal for Vitamin and Nutrition Research, 91(5-6), 500–506. doi:10.1024/0300-9831/a000649

Vale, F. B. C., Boroni, J. D., Geber, G. e., Antunes, E. M. G., Bretas, T., Lopes, G. P., & Geber, S. (2021). Effect of Tribulus terrestris in the treatment of female sexual dysfunction and clitoral vascularization. Results of a randomized study comparing two different dosage regimes. Journal of Sex & Marital Therapy, 47(7), 696–706. doi:10.1080/0092623x.2021.1938764

Walker, D., Bird, A., Flora, T., & O'Sullivan, B. (1992). Some effects of feeding Tribulus terrestris, Ipomoea lonchophylla and the seed of Abelmoschus ficulneus on fetal development and the outcome of pregnancy in sheep. Reproduction, Fertility and Development, 4(2). doi:10.1071/rd9920135

Zhu, W., Du, Y., Meng, H., Dong, Y., & Li, L. (2017). A review of traditional pharmacological uses, phytochemistry, and pharmacological activities of Tribulus terrestris. Chemistry Central Journal, 11(1). doi:10.1186/s13065-017-0289-x